How Artificial Sweeteners Destroy Your Gut

Zero calories often means zero nutrition and a host of potential problems
By Joseph Mercola
Joseph Mercola
Joseph Mercola
Dr. Joseph Mercola is the founder of An osteopathic physician, best-selling author, and recipient of multiple awards in the field of natural health, his primary vision is to change the modern health paradigm by providing people with a valuable resource to help them take control of their health. This article was originally published on
July 24, 2021 Updated: July 24, 2021

After years of investigation about the dangers of artificial sweeteners, I wrote a book, “Sweet Deception: Why Splenda, NutraSweet, and the FDA May Be Hazardous to Your Health,” and published it in 2006. Since then, I’ve been warning the world about the ever-growing evidence that artificial sweeteners can damage your health in many ways. Now, new research finds that gut microbiome damage from artificial sweeteners is even greater than was previously thought.

Scientists have found that three of the most popular artificial sweeteners, including sucralose (Splenda), aspartame (NutraSweet, Equal, and Sugar Twin), and saccharin (Sweet’n Low, Necta Sweet, and Sweet Twin) have a pathogenic effect on two types of gut bacteria.

Specifically, research using lab data was published in the International Journal of Molecular Sciences, which demonstrated these common sweeteners can trigger beneficial bacteria to become pathogenic and potentially increase your risk of serious health conditions. This is the first study that demonstrated how two types of beneficial bacteria can become diseased and invade the gut wall.

The bacteria studied were Escherichia coli (E. coli) and Enterococcus faecalis (E. faecalis). As early as 2008, researchers found that sucralose lowered your gut bacteria count by at least 47.4 percent and increased the pH level of your intestines. Another study found that sucralose had a metabolic effect on bacteria and could inhibit the growth of certain species.

Just 2 Cans of Diet Soda Can Alter Beneficial Bacteria

The current molecular research from Angelia Ruskin University found that when E. coli and E. faecalis became pathogenic, they killed Caco-2 cells that line the wall of the intestines. Much of the past research demonstrating a change in gut bacteria had used sucralose.

However, data from this study showed that a concentration from two cans of diet soft drinks, using any of the three artificial sweeteners, could significantly increase the ability of E. coli and E. faecalis to adhere to the Caco-2 cells and increase the development of bacterial biofilms.

When bacteria create a biofilm, it promotes the invasion of the intestinal cell wall. Biofilms make bacteria less sensitive to treatment and more likely to express virulence that causes disease. Each of the three sweeteners tested also triggered the bacteria to invade the Caco-2 cells, with one exception.

The researchers found that saccharin didn’t have a significant effect on E. coli invading the Caco-2 cells. Havovi Chichger, lead author and senior lecturer in Biomedical Science at Anglia Ruskin University, spoke about the results of the study in a press release:

“Our study is the first to show that some of the sweeteners most commonly found in food and drink—saccharin, sucralose and aspartame—can make normal and ‘healthy’ gut bacteria become pathogenic.”

“These changes could lead to our own gut bacteria invading and causing damage to our intestine, which can be linked to infection, sepsis and multiple-organ failure.”

Artificial Sweeteners Can Sabotage Your Diet Goals

Unfortunately, for many people, their sweet tooth has become an addiction, fueled by a food industry that continues to develop highly palatable, inexpensive, and ultra-processed foods loaded with sugar as well as artificial sweeteners. As such, the diet industry has become a cash-cow market for lab-created, low-calorie foods manufacturers promote for weight loss.

One study from George Washington University Milken Institute School of Public Health in 2017 found there was a 54 percent jump in adults who used low-calorie sweeteners from 1999 to 2012. This represented 41.4 percent of all adults in the United States at that time, or 129.5 million people. By 2020, the number had jumped to 141.18 million, which represented 42.6 percent of the population.

It appears that the jump in adults using low-calorie sweeteners that occurred from 1999 to 2012 has remained steady through 2020. This may be due in part to the growing evidence that low-calorie sweeteners, such as Splenda, are a large contributor to the growing number of individuals who are overweight and obese.

As the incidence of obesity and obesity-related health conditions continues to skyrocket, manufacturers seek out “perfectly engineered food” to drive sales and consumption.

Consequently, the obesity epidemic is one of the most important global public health challenges today, associated with 4.7 million premature deaths worldwide in 2017. Recent research suggests artificial sweeteners may contribute to a greater range of health conditions than we have thus far identified.

Metabolic Effects of Zero Calorie Sweeteners

It’s important to recognize that even though artificial sweeteners have very few or no calories, they are still metabolically active. The New York Times reported that the FDA announced it was banning saccharin in foods and beverages in 1977 because it was linked to the development of malignant bladder tumors in laboratory animals.

However, the FDA later approved the use of saccharin saying “more than 30 human studies demonstrated that the results found in rats were not relevant to humans, and that saccharin is safe for human consumption.”

But just because the FDA has approved something, it doesn’t mean it’s good for you. Scientists have explained that many studies have linked artificial sweeteners to an increased risk for obesity, insulin resistance, Type 2 diabetes, and metabolic syndrome. A paper published in Physiology and Behavior presented three mechanisms by which artificial sweeteners promote metabolic dysfunction:

They interfere with learned responses that contribute to glucose control and energy homeostasis; destroy gut microbiota and induce glucose intolerance; and they interact with sweet-taste receptors expressed throughout the digestive system that play a role in glucose absorption and trigger insulin secretion.

As past and recent research has demonstrated, artificial sweeteners have a significantly different effect on your gut microbiome than sugar. Sugar is detrimental because it tends to feed harmful microbes, yet the effects of artificial sweeteners may be worse, as they are downright toxic to gut bacteria.

One animal study published in the journal Molecules analyzed six artificial sweeteners including saccharin, sucralose, aspartame, neotame, advantame, and acesulfame potassium-K. The data showed they all caused DNA damage in, and interfered with, the normal and healthy activity of gut bacteria.

Diet Drinks Increase the Risk of an Early Death

One 20-year, population-based study of 451,743 people from 10 European countries discovered there was also an association between artificially sweetened drinks and mortality. The researchers excluded participants who had previously had cancer, stroke, or diabetes.

At the final tally, 71.1 percent of the participants in the study were women. The results showed that there was a higher all-cause mortality in people who drank two or more glasses each day of soft drinks, whether they were sugar-sweetened or artificially sweetened.

The researchers measured one glass as equivalent to 250 milliliters (8.4 ounces), which is less than the standard 330 milliliters (11.3 ounces) per can sold in Europe. In other words, the results of the study were based on less than two cans of soda each day.

The researchers found 43.2 percent of deaths were from cancers, 21.8 percent from circulatory disease, and 2.9 percent from digestive disorders. Compared to those who drank fewer soft drinks (less than one per month) those drinking two or more per day were more likely to be young, smokers, and physically active.

The data showed there was a link between artificially sweetened soft drinks and death from circulatory diseases and an association between sugar-sweetened soft drinks and death from digestive diseases. This suggests that policies aimed to cut or reduce sugar consumption may have disastrous consequences when manufacturers reformulate their products using artificial sweeteners.

More Health Damage Associated With Artificial Sweeteners

This same study also found a link between drinking soft drinks and Parkinson’s Disease “with positive nonsignificant associations found for sugar-sweetened and artificially sweetened soft drinks.”

Aspartame is another artificial sweetener that has been studied in the past decades. In one study, researchers asked healthy adults to consume a high-aspartame diet for eight days, followed by a two-week washout and then a low-aspartame diet for eight days.

During the high-aspartame period, individuals suffered from depression, headache, and poor mood. They performed worse on spatial orientation tests, which indicated aspartame had a significant effect on neurobehavioral health.

A second study evaluated whether people with diagnosed mood disorders were more vulnerable to the effects of aspartame. Researchers included 40 individuals with unipolar depression and those without any history of psychiatric disorder. The study was stopped after 13 completed the intervention because of the severity of the reactions.

Mice fed aspartame-laced drinking water developed symptoms of metabolic syndrome and another animal study found that aspartame had a negative effect on insulin tolerance and influenced gut microbial composition.

A further animal study determined that sucralose affected animal liver, “indicating toxic effects on regular ingestion.” The finding suggests “sucralose should be taken with caution to avoid hepatic damage.”

Scientists have found a long list of symptoms associated with consuming sucralose. These have included migraine headaches, raised risk of Type 2 diabetes, and enlargement of the liver and kidneys.

Sugar Alternative Has a Unique Action on Blood Sugar

There are several plant-based sugar substitutes, including stevia, Lo Han Kuo, and allulose. Stevia is a sweet herb from the South American stevia plant. It’s sold as a supplement and can be used to sweeten most dishes and drinks.

Lo Han Kuo is similar to stevia but a bit more expensive. Another natural option is allulose. Although the market in Japan is significant, it’s relatively unknown in the West. Allulose is found in small quantities in some fruits and was given a generally regarded as safe (GRAS) food designation by the FDA.

Researchers have said the compound has an energy value of “effectively zero,” which suggests this rare sugar may be useful as a sweetener for obese people to aid in weight reduction.

In addition to contributing little to no calories, allulose elicits a physiological response that may help to lower blood glucose, reduce abdominal fat, and reduce fat accumulation around the liver. Read more about this natural compound in “Can This Natural Sweetener Lower Blood Sugar?

Dr. Joseph Mercola is the founder of An osteopathic physician, best-selling author, and recipient of multiple awards in the field of natural health, his primary vision is to change the modern health paradigm by providing people with a valuable resource to help them take control of their health. This article was originally published on

Joseph Mercola
Joseph Mercola
Dr. Joseph Mercola is the founder of An osteopathic physician, best-selling author, and recipient of multiple awards in the field of natural health, his primary vision is to change the modern health paradigm by providing people with a valuable resource to help them take control of their health. This article was originally published on