In what was possibly the most shared moment of the awards ceremony, she rubbed against his chair, forcing him to take his arm down.
Caught by surprise, DiCaprio then made a face at her as she moved toward the stage to accept her award.
The 41-year-old later told Entertainment Tonight: “Oh lord—that’s trending, huh?”
“I just didn’t know what was passing me—that’s all!” he added.
Gaga won Best Actress in a Supporting Role in a Limited Series for her role in “American Horror Story: Hotel,” while DiCaprio won Best Actor for his role in “The Revenant.”